Early detection of oxacillin-resistant staphylococcal strains with hypertonic broth diluent for microdilution panels
نویسندگان
چکیده
منابع مشابه
Evaluation of MicroScan rapid gram-positive panels for detection of oxacillin-resistant staphylococci.
The ability of MicroScan rapid panels (Baxter MicroScan, West Sacramento, Calif.) to detect oxacillin resistance in 92 clinical isolates of Staphylococcus aureus and 103 coagulase-negative staphylococci was evaluated by comparing results with those of MicroScan 24-h MIC panels and, to resolve discrepancies, oxacillin agar screening. Both panels were interpreted by the MicroScan WalkAway-96 syst...
متن کاملPerformance of eight methods, including two new rapid methods, for detection of oxacillin resistance in a challenge set of Staphylococcus aureus organisms.
Using a set of 55 Staphylococcus aureus challenge organisms, we evaluated six routine methods (broth microdilution, disk diffusion, oxacillin agar screen, MicroScan conventional panels, MicroScan rapid panels, and Vitek cards) currently used in many clinical laboratories and two new rapid methods, Velogene and the MRSA-Screen, that require less than a day to determine the susceptibility of S. a...
متن کاملPhenotypic Changes of Oxacillin Resistance in Methicillin-resistant Staphylococcus aureus Strains by Successive Passaging
Background and purpose: Rotational use of antibiotics can decrease antibiotic resistance in Staphylococcus aureus strains. The present study aimed to evaluate the alteration of oxacillin resistance in methicillin-resistant Staphylococcus aureus (MRSA) strain carrying SCCmec III cassette in long-term evolutionary experiments in an antibiotic-free medium. Materials and methods: In a cross-sectio...
متن کاملBranched-DNA assay for detection of the mecA gene in oxacillin-resistant and oxacillin-sensitive staphylococci.
The identification of methicillin-resistant staphylococcus isolates in the clinical laboratory has typically been performed by using methods that detect phenotypic expression of resistance determinants. However, these methods may be difficult to interpret and some isolates do not express resistance until selective pressure is administered. Assays that detect genetic determinants are not subject...
متن کاملComparison of cation-adjusted Mueller-Hinton broth with Iso-Sensitest broth for the NCCLS broth microdilution method.
Comparison of MIC results obtained in different parts of the world is currently difficult because of variations in methods. In this study, cation-adjusted Mueller-Hinton broth, the NCCLS-recommended medium, was compared with Iso-Sensitest broth, which is widely used in Europe. Microbroth dilution testing, using the NCCLS procedure, was performed on 124 Gram-positive (staphylococci and enterococ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1984
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.19.4.473-476.1984